Gene expression analysis in citrus reveals the role of gibberellins on photosynthesis and stress.
Identifieur interne : 002045 ( Main/Exploration ); précédent : 002044; suivant : 002046Gene expression analysis in citrus reveals the role of gibberellins on photosynthesis and stress.
Auteurs : Laura Huerta [Espagne] ; Javier Forment ; José Gadea ; Carmen Fagoaga ; Leandro Pe A ; Miguel A. Pérez-Amador ; José Luis García-MartínezSource :
- Plant, cell & environment [ 1365-3040 ] ; 2008.
English descriptors
- KwdEn :
- Citrus (drug effects), Citrus (genetics), Citrus (metabolism), Gene Expression Profiling, Gene Expression Regulation, Plant, Genes, Plant, Gibberellins (pharmacology), Mixed Function Oxygenases (metabolism), Oligonucleotide Array Sequence Analysis, Photosynthesis (drug effects), Plant Growth Regulators (pharmacology), Plants, Genetically Modified (drug effects), Plants, Genetically Modified (genetics), Plants, Genetically Modified (metabolism), RNA, Plant (genetics), Reverse Transcriptase Polymerase Chain Reaction.
- MESH :
- chemical , genetics : RNA, Plant.
- chemical , metabolism : Mixed Function Oxygenases.
- chemical , pharmacology : Gibberellins, Plant Growth Regulators.
- drug effects : Citrus, Photosynthesis, Plants, Genetically Modified.
- genetics : Citrus, Plants, Genetically Modified.
- metabolism : Citrus, Plants, Genetically Modified.
- Gene Expression Profiling, Gene Expression Regulation, Plant, Genes, Plant, Oligonucleotide Array Sequence Analysis, Reverse Transcriptase Polymerase Chain Reaction.
Abstract
The effect of gibberellins (GA) on internode transcriptome was investigated in transgenic Carrizo citrange (Citrus sinensis x Poncirus trifoliata) plants overexpressing endogenous CcGA20ox1 (encoding a GA biosynthetic gene), and in non-transformed explants treated with GA(3), using a citrus cDNA microarray. Substantial modulation of gene expression was found in sense CcGA20ox plants. Extensive up-regulation of genes involved in photosynthesis and carbon utilization, and down-regulation of those involved in protein synthesis and ribosome biogenesis were shown for the first time in plants with higher GA content. Importantly, increase of net photosynthesis in attached leaves was also demonstrated. Expression of other genes belonging to functional groups not reported previously to be regulated by GA (mainly abiotic and biotic stresses, and cuticle biosynthesis), and genes involved in cell division and cell wall architecture were also differentially expressed. Culture of citrus explants for 24 h in GA(3) solution produced much lower changes in the transcriptome compared with CcGA20ox plants (1.6% versus 16%, respectively, of total genes in the microarray), suggesting that most of the changes observed in CcGA20ox plants were a consequence of a long-standing GA effect. Interestingly, genes related to abiotic and biotic stresses were similarly modulated in transgenics and GA(3)-treated explants.
DOI: 10.1111/j.1365-3040.2008.01870.x
PubMed: 18684239
Affiliations:
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Pérez-Amador</name></author><author><name sortKey="Garcia Martinez, Jose Luis" sort="Garcia Martinez, Jose Luis" uniqKey="Garcia Martinez J" first="José Luis" last="García-Martínez">José Luis García-Martínez</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2008">2008</date><idno type="RBID">pubmed:18684239</idno><idno type="pmid">18684239</idno><idno type="doi">10.1111/j.1365-3040.2008.01870.x</idno><idno type="wicri:Area/PubMed/Corpus">000A17</idno><idno type="wicri:Area/PubMed/Curation">000A17</idno><idno type="wicri:Area/PubMed/Checkpoint">000A17</idno><idno type="wicri:Area/Ncbi/Merge">000872</idno><idno type="wicri:Area/Ncbi/Curation">000872</idno><idno type="wicri:Area/Ncbi/Checkpoint">000872</idno><idno type="wicri:Area/Main/Merge">002103</idno><idno type="wicri:Area/Main/Curation">002045</idno><idno type="wicri:Area/Main/Exploration">002045</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Gene expression analysis in citrus reveals the role of gibberellins on photosynthesis and stress.</title><author><name sortKey="Huerta, Laura" sort="Huerta, Laura" uniqKey="Huerta L" first="Laura" last="Huerta">Laura Huerta</name><affiliation wicri:level="1"><nlm:affiliation>Instituto de Biología Molecular y Celular de Plantas (IBMCP), Universidad Politécnica de Valencia-CSIC, Valencia, Spain.</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Instituto de Biología Molecular y Celular de Plantas (IBMCP), Universidad Politécnica de Valencia-CSIC, Valencia</wicri:regionArea><wicri:noRegion>Valencia</wicri:noRegion></affiliation></author><author><name sortKey="Forment, Javier" sort="Forment, Javier" uniqKey="Forment J" first="Javier" last="Forment">Javier Forment</name></author><author><name sortKey="Gadea, Jose" sort="Gadea, Jose" uniqKey="Gadea J" first="José" last="Gadea">José Gadea</name></author><author><name sortKey="Fagoaga, Carmen" sort="Fagoaga, Carmen" uniqKey="Fagoaga C" first="Carmen" last="Fagoaga">Carmen Fagoaga</name></author><author><name sortKey="Pe A, Leandro" sort="Pe A, Leandro" uniqKey="Pe A L" first="Leandro" last="Pe A">Leandro Pe A</name></author><author><name sortKey="Perez Amador, Miguel A" sort="Perez Amador, Miguel A" uniqKey="Perez Amador M" first="Miguel A" last="Pérez-Amador">Miguel A. Pérez-Amador</name></author><author><name sortKey="Garcia Martinez, Jose Luis" sort="Garcia Martinez, Jose Luis" uniqKey="Garcia Martinez J" first="José Luis" last="García-Martínez">José Luis García-Martínez</name></author></analytic><series><title level="j">Plant, cell & environment</title><idno type="eISSN">1365-3040</idno><imprint><date when="2008" type="published">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Citrus (drug effects)</term><term>Citrus (genetics)</term><term>Citrus (metabolism)</term><term>Gene Expression Profiling</term><term>Gene Expression Regulation, Plant</term><term>Genes, Plant</term><term>Gibberellins (pharmacology)</term><term>Mixed Function Oxygenases (metabolism)</term><term>Oligonucleotide Array Sequence Analysis</term><term>Photosynthesis (drug effects)</term><term>Plant Growth Regulators (pharmacology)</term><term>Plants, Genetically Modified (drug effects)</term><term>Plants, Genetically Modified (genetics)</term><term>Plants, Genetically Modified (metabolism)</term><term>RNA, Plant (genetics)</term><term>Reverse Transcriptase Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>RNA, Plant</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Mixed Function Oxygenases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Gibberellins</term><term>Plant Growth Regulators</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Citrus</term><term>Photosynthesis</term><term>Plants, Genetically Modified</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Citrus</term><term>Plants, Genetically Modified</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Citrus</term><term>Plants, Genetically Modified</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Expression Profiling</term><term>Gene Expression Regulation, Plant</term><term>Genes, Plant</term><term>Oligonucleotide Array Sequence Analysis</term><term>Reverse Transcriptase Polymerase Chain Reaction</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The effect of gibberellins (GA) on internode transcriptome was investigated in transgenic Carrizo citrange (Citrus sinensis x Poncirus trifoliata) plants overexpressing endogenous CcGA20ox1 (encoding a GA biosynthetic gene), and in non-transformed explants treated with GA(3), using a citrus cDNA microarray. Substantial modulation of gene expression was found in sense CcGA20ox plants. Extensive up-regulation of genes involved in photosynthesis and carbon utilization, and down-regulation of those involved in protein synthesis and ribosome biogenesis were shown for the first time in plants with higher GA content. Importantly, increase of net photosynthesis in attached leaves was also demonstrated. Expression of other genes belonging to functional groups not reported previously to be regulated by GA (mainly abiotic and biotic stresses, and cuticle biosynthesis), and genes involved in cell division and cell wall architecture were also differentially expressed. Culture of citrus explants for 24 h in GA(3) solution produced much lower changes in the transcriptome compared with CcGA20ox plants (1.6% versus 16%, respectively, of total genes in the microarray), suggesting that most of the changes observed in CcGA20ox plants were a consequence of a long-standing GA effect. Interestingly, genes related to abiotic and biotic stresses were similarly modulated in transgenics and GA(3)-treated explants.</div></front></TEI><affiliations><list><country><li>Espagne</li></country></list><tree><noCountry><name sortKey="Fagoaga, Carmen" sort="Fagoaga, Carmen" uniqKey="Fagoaga C" first="Carmen" last="Fagoaga">Carmen Fagoaga</name><name sortKey="Forment, Javier" sort="Forment, Javier" uniqKey="Forment J" first="Javier" last="Forment">Javier Forment</name><name sortKey="Gadea, Jose" sort="Gadea, Jose" uniqKey="Gadea J" first="José" last="Gadea">José Gadea</name><name sortKey="Garcia Martinez, Jose Luis" sort="Garcia Martinez, Jose Luis" uniqKey="Garcia Martinez J" first="José Luis" last="García-Martínez">José Luis García-Martínez</name><name sortKey="Pe A, Leandro" sort="Pe A, Leandro" uniqKey="Pe A L" first="Leandro" last="Pe A">Leandro Pe A</name><name sortKey="Perez Amador, Miguel A" sort="Perez Amador, Miguel A" uniqKey="Perez Amador M" first="Miguel A" last="Pérez-Amador">Miguel A. Pérez-Amador</name></noCountry><country name="Espagne"><noRegion><name sortKey="Huerta, Laura" sort="Huerta, Laura" uniqKey="Huerta L" first="Laura" last="Huerta">Laura Huerta</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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